膜杰作
Star Staining
产品描述（Product Details）
This product is derived from Streptococcus pyogenes and expressed in E. coli, it is an IgG-specific cysteine protease that digests antibodies at a single amino acid site below the hinge, generating homogenous F(ab’)2 and Fc fragments.
应用说明（Application）
- IdeS cleaves IgG to produce F(ab')2 and Fc fragments at specific site.
单位定义（Unit Definition）
One unit digests ≥ 95% of 1 μg human IgG when incubated in 10 mM sodium phosphate, 137 mM NaCl, 2.7 mM KCl, pH7.4 at 37°C for 30min.
质量控制（Quility Control）
Less than 1.0 EU per μg by the LAL method. >95% as determined by SDS-PAGE.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
存储（Storage）
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
活性（Bioactivity）
50/100unit Ides digests ≥ 95% of 100 µg human IgG when incubated in PBS pH 7.4 at 37℃ for 30min.
50/100unit Ides digests ≥ 95% of 100 µg human IgG when incubated in PBS pH 7.4 at 37℃ for 30min.
