膜杰作
Star Staining
分子别名（Synonym）
HGF,HPTA,SF
表达区间及表达系统（Source）
Human HGF, His Tag, premium grade (HGF-H52H3) is expressed from human 293 cells (HEK293). It contains AA Gln 32 - Ser 728 (Accession # P14210-1).
Predicted N-terminus: Gln 32
It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The mature form of HGF is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. The protein has a calculated MW of 81.6 kDa (α chain 53.7 kDa and β chain 27.9 kDa). The protein migrates as 80-90 kDa (alpha & Beta chain), 58-63 kDa (alpha chain), 33 kDa (Beta chain) when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 0.1 EU per μg by the LAL method.
无菌（Sterility）
The sterility testing was performed by membrane filtration method.
支原体（Mycoplasma）
Negative.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human HGF, His Tag, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human HGF, His Tag, premium grade (Cat. No. HGF-H52H3) is more than 90% and the molecular weight of this protein is around 90-105 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-Organoid Culture
Human EGF (Cat. No. EGF-H52H3), Noggin (Cat. No. NON-H5257), R-spondin1 (Cat. No. RS6-H4220), FGF7 (Cat. No. FG7-H52H5), FGF10 (Cat. No. FG0-H5145), HGF (Cat. No. HGF-H52H3) actively support liver ductal organoid growth.
活性（Bioactivity）-Bioactivity CELL BASE
Human HGF Protein, His Tag, premium grade (Cat. No. HGF-H52H3) stimulates the secretion of IL-11 by Saos-2 cells. The EC50 for this effect is 0.654 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-ELISA
Immobilized Human HGF, His Tag, premium grade (Cat. No.HGF-H52H3 ) at 2 μg/mL (100 μL/well) can bind Human HGF R, Fc Tag (Cat. No. MET-H5256) with a linear range of 1-39 ng/mL (QC tested).
Protocol
活性（Bioactivity）-SPR
Human HGF R, Fc Tag (Cat. No. MET-H5256) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human HGF, His Tag, premium grade (Cat. No. HGF-H52H3) with an affinity constant of 0.486 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Loaded Human HGF R, Fc Tag (Cat. No. MET-H5256) on Protein A Biosensor, can bind Human HGF, His Tag, premium grade (Cat. No. HGF-H52H3) with an affinity constant of 3.29 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
背景（Background）
Hepatocyte growth factor (HGF) is a paracrine cellular growth, motility and morphogenic factor. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. In addition, HGF has been implicated in a variety of cancers, including of the lungs, pancreas, thyroid, colon, and breast.
