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 >  Protein>ROR1 >RO1-HF258

FITC-Labeled Human ROR1 Protein, His Tag

分子别名(Synonym)

ROR1,NTRKR1

表达区间及表达系统(Source)

FITC-Labeled Human ROR1, His Tag (RO1-HF258) is expressed from human 293 cells (HEK293). It contains AA Gln 30 - Glu 403 (Accession # Q01973-1). It is the FITC labeled form of Human ROR1, His Tag.

Predicted N-terminus: Gln 30

Request for sequence

蛋白结构(Molecular Characterization)

ROR1 Structure

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 43.9 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

偶联(Conjugate)

FITC

Excitation source: 488 nm spectral line, argon-ion laser

Excitation Wavelength: 488 nm

Emission Wavelength: 535 nm

标记(Labeling)

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.

蛋白标记度(Protein Ratio)

The FITC to protein molar ratio is 1.5-3.5.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

ROR1 SDS-PAGE

FITC-Labeled Human ROR1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

ROR1 ELISA

Immobilized Anti-ROR1 Antibody, Human IgG1 at 1 μg/mL (100 μL/well) can bind FITC-Labeled Human ROR1, His Tag (Cat. No. RO1-HF258) with a linear range of 0.078-1.25 μg/mL (QC tested).

Protocol

 

活性(Bioactivity)-FACS

ROR1 FACS

2e5 of anti-ROR1 CAR-293 cells were stained with 100 μL of 10 μg/mL of FITC-Labeled Human ROR1, His Tag (Cat. No.RO1-HF258) and negative control protein respectively, FITC signal was used to evaluate the binding activity (QC tested).

Protocol

 

背景(Background)

Tyrosine-protein kinase transmembrane receptor ROR1 is also known as Neurotrophic tyrosine kinase, receptor-related 1 (NTRKR1), which belongs to the protein kinase superfamily or tyr protein kinase family or ROR subfamily. ROR1 contains 1 FZ (frizzled) domain, 1 Ig-like C2-type (immunoglobulin-like) domain, 1 kringle domain, 1 protein kinase domain. ROR1 is expressed at high levels during early embryonic development. The expression levels drop strongly around day 16 and there are only very low levels in adult tissues. Isoform Short is strongly expressed in fetal and adult CNS and in a variety of human cancers, including those originating from CNS or PNS neuroectoderm. ROR1 could interact with casein kinase 1 epsilon (CK1ε) to activate phosphoinositide 3-kinase-mediated AKT phosphorylation and cAMP-response-element-binding protein (CREB), which was associated with enhanced tumor-cell growth.

文献引用(Citations)

 

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ROR1靶点信息
英文全称:Inactive tyrosine-protein kinase transmembrane receptor ROR1
中文全称:受体酪氨酸激酶样孤儿受体1
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:12详情
最高研发阶段:临床三期
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