| Items | Size （2mg） | Size（5mg X 2） |
| Particle size | 2 μm | 2 μm |
| Coupled amount of protein | >1700 pmol (50 μg) CD33 / mg Beads | >1700 pmol (50 μg) CD33 / mg Beads |
| Capacity | >267 pmol (40 μg) antibody / mg beads | >267 pmol (40 μg) antibody / mg beads |
| Formulation | PBS, pH7.4, with 10% Trehalose | PBS, pH7.4, with 10% Trehalose |
| Product Format | Powder | Powder |
| Reconstitution | 2 mL ultrapure water (1 mg beads / mL) | Reconstitute 5 mg / bottle Beads with 5 mL ultrapure water (1 mg beads / mL) |
| Concentration | 1 mg beads / mL | 1 mg beads / mL |
Background/背景
The biotinylated CD33 protein was conjugated to streptavidin magnetic beads. This pre-coupled magnetic bead product can capture the anti-CD33 antibody from various assay systems. The beads are in uniform size, narrow size distribution with large surface area and unique surface coating, which can help you get the best performance and highly reproducible results. This CD33 coupled magnetic beads will bring great convenience with minimum non-specific binding and developed protocols. This ready-to-use product could greatly save your time and hassle.
Application/应用
This product is intended for immunocapture, biopanning and flow cytometry. This product is produced non-sterile.
Reconstitution/重构方法
See Certificate of Analysis for details of reconstitution instruction and specific concentration.
Storage/存储
Upon receipt, please store the Beads at -20℃. The shelf life is 1 year at -20 ℃.
Please avoid more than 3 freeze-thaw cycles. Immediate use after reconstitution is highly recommended.
Assay Principles/原理
The conjugation was achieved by means of the binding between streptavidin and biotin. Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10-14 mol/L. Thus, the binding of streptavidin and biotin is irreversible. Our CD33 pre-coupled beads could capture anything binding to CD33, and make the following testing easy, such as immunocapture, biopanning and flow cytometry.
Application Method:
a) Reconstitute the Beads following the COA. Wash and re-suspended the beads to a certain concentration by adding your dilution buffer.
b) Add the prepared beads to your samples.
c) Beads can be separated from your samples afterwards using a magnetic plate.
Typical Data/典型数据
Immobilized 53.7 μg CD33 protein to 1 mg Beads, can bind the Anti-CD33 Antibody with an EC50 of 0.7794 μg/mL (QC tested).
Protocol
