Recombinant Proteins iso9001 ISO13485
搜索
 >  Beads>Siglec-3 >MBC-K008

Human CD33-coupled Magnetic Beads

ItemsSize (2mg)Size(5mg X 2)
Particle size2 μm2 μm
Coupled amount of protein>1700 pmol (50 μg) CD33 / mg Beads>1700 pmol (50 μg) CD33 / mg Beads
Capacity>267 pmol (40 μg) antibody / mg beads>267 pmol (40 μg) antibody / mg beads
FormulationPBS, pH7.4, with 10% TrehalosePBS, pH7.4, with 10% Trehalose
Product FormatPowderPowder
Reconstitution2 mL ultrapure water (1 mg beads / mL)Reconstitute 5 mg / bottle Beads with 5 mL ultrapure water (1 mg beads / mL)
Concentration1 mg beads / mL1 mg beads / mL

Background/背景

The biotinylated CD33 protein was conjugated to streptavidin magnetic beads. This pre-coupled magnetic bead product can capture the anti-CD33 antibody from various assay systems. The beads are in uniform size, narrow size distribution with large surface area and unique surface coating, which can help you get the best performance and highly reproducible results. This CD33 coupled magnetic beads will bring great convenience with minimum non-specific binding and developed protocols. This ready-to-use product could greatly save your time and hassle.

Application/应用

This product is intended for immunocapture, biopanning and flow cytometry. This product is produced non-sterile.

Reconstitution/重构方法

See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage/存储

Upon receipt, please store the Beads at -20℃. The shelf life is 1 year at -20 ℃.

Please avoid more than 3 freeze-thaw cycles. Immediate use after reconstitution is highly recommended.

Assay Principles/原理

The conjugation was achieved by means of the binding between streptavidin and biotin. Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10-14 mol/L. Thus, the binding of streptavidin and biotin is irreversible. Our CD33 pre-coupled beads could capture anything binding to CD33, and make the following testing easy, such as immunocapture, biopanning and flow cytometry.

Application Method:

a) Reconstitute the Beads following the COA. Wash and re-suspended the beads to a certain concentration by adding your dilution buffer.

b) Add the prepared beads to your samples.

c) Beads can be separated from your samples afterwards using a magnetic plate.

 

Typical Data/典型数据

Biotinylated Human ELISA

Immobilized 53.7 μg CD33 protein to 1 mg Beads, can bind the Anti-CD33 Antibody with an EC50 of 0.7794 μg/mL (QC tested).

Protocol

前沿进展

 
货号/价格
开发建议
文档
联系电话:
400-682-2521(全国)
010-57737274(北京)
021-26136428(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
techsupport@acrobiosystems.com
Siglec-3靶点信息
英文全称:Myeloid cell surface antigen CD33
中文全称:髓系细胞表面抗原CD33
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:20详情
最高研发阶段:批准上市
查看更多信息
推荐会议
查看全部会议

消息提示

请输入您的联系方式,再点击提交!

确定