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Magnetic Beads™ Streptavidin

ItemsSize (5mg)Size(5mg X 2)
Particle size2 μm2 μm
Physical appearancePowder mixturePowder mixture
Amount of Coupled Protein>1000 pmol/mg Beads>1000 pmol/mg Beads
Binding Capacity>300 pmol (45 μg) antibody/mg Beads>300 pmol (45 μg) antibody/mg Beads
FormulationPBS, pH7.4, with 10% TrehalosePBS, pH7.4, with 10% Trehalose
Reconstitution5 mL sterile deionized water (1 mg beads/mL)5 mL sterile deionized water (1 mg beads/mL)

背景(Background)

The streptavidin magnetic beads are uniform size, narrow size distribution with large surface area and unique surface coating, it is easy to capture the biotinylated proteins or other molecules, and the bounded protein have no activity lost, can help us get the best performance and highly reproducible results.

应用说明(Application)

This product is intended for immunocapture, biopanning and flow cytometry. This product is produced non-sterile.

重构方法(Reconstitution)

See Certificate of Analysis (CoA) for detailed instruction.

存储(Storage)

This product is stable for 1 year when stored at -20 °C.

Please avoid more than 3 freeze-thaw cycles. Immediate use after reconstitution is highly recommended.

原理(Assay Principles)

Application Method: a) Reconstitute the beads with ultrapure water to 1mg/mL, and wash the beads for three times. b) Dilute the biotinalytated protein or antibodies with Assay Buffer, and add to the beads, incubate for 60 minutes at room temperature in a rotary mixer. c) Wash the beads for four times, and add assay buffer to beads for next experiment.

Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10−14 mol/L, the Biotinylated protein can bind to the SA beads irreversibly. The Magenetic beads are pre-coupled with Streptavidin tetramer protein and blocked with BSA, it is a ready-to-use beads, which can be used for binding biotinylated proteins and antibodies.

 

典型数据-Typical Data

Streptavidin ELISA

Immobilized 40 μg SARS-CoV-2 S1 protein to 1mg Beads add sample Anti-SARS-CoV-S protein RBD Antibody, detection Goat anti-Human IgG Fc Secondary Antibody, PE, with an EC50 0.1750 μg/mL (Routinely tested).

Protocol

Streptavidin ELISA

Freeze-thaw stability test. Immobilized 40 μg SARS-CoV-2 S1 protein to 1mg Beads, after different freeze-thaw cycles, add sample Anti-SARS-CoV-S protein RBD Antibody, detection Goat anti-Human IgG Fc Secondary Antibody, PE, showed little deviation from the unfreeze-thaw sample (RSD<10%). Three freeze-thaw cycles were performed (Routinely tested).

Protocol

Streptavidin ELISA

Accelerated stability test. Immobilized 40 μg SARS-CoV-2 S1 protein to 1mg Beads, after placing the lyophilized beads at 37°C for 7 daysadd sample Anti-SARS-CoV-S protein RBD Antibody, detection Goat anti-Human IgG Fc Secondary Antibody, PE, showed little deviation from the unfreeze-thaw sample (RSD<10%). Data were measured on day 0, 3, 7 respectively (Routinely tested).

Protocol

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