Human CD28H, His Tag (CDH-H52H3) is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Gly 150 (Accession # Q96BF3-1).
Predicted N-terminus: Leu 23
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 15.9 kDa. The protein migrates as 22-32 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>90% as determined by reduced SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human CD28H, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human CD28H, His Tag (Cat. No. CDH-H52H3) at 5 μg/mL (100 μL/well) can bind Biotinylated Human B7-H7, Fc Tag, Avi Tag (Cat. No. B77-H82F5) with a linear range of 10-156 ng/mL (QC tested).
Immobilized Human CD28H, His Tag (Cat. No. CDH-H52H3) at 5 μg/mL (100 μL/well) can bind Human B7-H7, Fc Tag (Cat. No. B77-H5257) with a linear range of 1-78 ng/mL (Routinely tested).
CD28 homolog (CD28H), also called transmembrane and immunoglobulin domain containing 2 (TMIGD2) and IGPR-1,is encoded by the TMIGD2 gene. CD28H is constitutively expressed on naive T and NK cells. After interaction of CD28 homolog with B7 homologue,the peripheral effector and memory T cells can be activated and proliferated by Akt-dependent signalling cascade. Plays a role in cell-cell interaction, cell migration, and angiogenesis. Through interaction with HHLA2, costimulates T-cells in the context of TCR-mediated activation. Enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade.