5e5 of anti-CD19 CAR-293 cells were stained with 100 μL of 10 μg/mL of AF555-Labeled Human CD19 (20-291), His Tag (Cat. No.CD9-HA2H7), and negative control protein respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). AF555 signal was used to evaluate the binding activity (QC tested).
5e5 of PBMCs were stained with AF555-Labeled Human CD19 (20-291), His Tag (Cat. No.CD9-HA2H7) and anti-CD3 antibody, washed and then analyzed with FACS. FITC signal was used to evaluate the expression of CD3+ T cells in PBMCs, and AF555 signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).
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