【靶点聚焦】CD40激动剂联合治疗是否为一剂良药？

CD40Ligand（activetrimer）产品列表

验证数据

★CD40 Ligand三聚体结构经MALS验证

Thepurity of Human CD40 Ligand, Fc Tag (active trimer) (MALS verified)(Cat. No. CDL-H5269)is more than 90% in HP-SEC, and the molecular weight of this proteinis around 160-180 kDa verified by SEC-MALS.

★高生物活性经ELISA/SPR/BLI/Cell based assay验证

>>>ELISA

Immobilized Human CD40, His Tag (Cat. No. CD0-H5228) at 1 μg/mL (100 μL/well)can bind Human CD40 Ligand, Fc Tag (Cat. No. CDL-H5267) with a linearrange of 0.2-8 ng/mL (QC tested).

>>>BLI

Loaded Human CD40, Fc Tag (Cat. No. CD0-H5253) on Protein A Biosensor, canbind Human CD40 Ligand, His,Flag Tag (Cat. No. CDL-H52Db) with anaffinity constant of 1.58 nM as determined in BLI assay (ForteBioOctet Red96e) (Routinely tested).

>>>Cell based assay

HumanCD40 Ligand, His,Flag Tag (Cat. No. CDL-H52Db) stimulates secretionof IL-6 by human B cells. The ED50 for this effect is 18.76-19.45ng/mL in the presence of 5 ng/mL of Recombinant Human IL‑4(Routinely tested).

Neutralization assay shows that the cytokine secretion effect of Human CD40 Ligand,His,Flag Tag (Cat. No. CDL-H52Db) was inhibited by increasingconcentration of anti-CD40L mAb. The concentration of CD40L used is100 ng/mL. The EC50 is 0.215 μg/mL (Routinely tested).

#更多免疫检查点蛋白 

#更多双抗热门靶点蛋白