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FITC-Labeled Human BCMA / TNFRSF17 Protein, Fc Tag DMF Filed

分子别名(Synonym)

TNFRSF17,CD269,BCM,BCMA

表达区间及表达系统(Source)

FITC-Labeled Human BCMA, Fc Tag (Cat. No. BCA-HF254) is expressed from human HEK293 cells. It contains AA Met 1 - Ala 54 (Accession # Q02223-1). It is the FITC labeled form of Human BCMA, Fc Tag (Cat. No. BC7-H5254).

Predicted N-terminus: Met 1

蛋白结构(Molecular Characterization)

Online(Met 1 - Ala 54) Q02223-1

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 32.0 kDa. As a result of glycosylation, the protein migrates as 35-44 kDa under reducing (R) condition, and 60-75 kDa under non-reducing (NR) condition (SDS-PAGE).

Conjugate

FITC

Excitation source: 488 nm spectral line, argon-ion laser

Excitation Wavelength: 488 nm

Emission Wavelength: 535 nm

标记(Labeling)

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular seive treatment during purification process.

蛋白标记度(FITC:Protein Ratio)

The FITC to protein molar ratio is 1.5-3.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>92% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 12 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

FITC-Labeled Human BCMA Protein, Fc Tag (Cat. No. BCA-HF254) SDS-PAGE gel

FITC-Labeled Human BCMA Protein, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human BAFF, Fc Tag (Cat. No. BAF-H4268) at 10 μg/mL (100 μL/well) can bind FITC-Labeled Human BCMA, Fc Tag (Cat. No. BCA-HF254) with a linear range of 0.04-1.25 μg/mL (Ex.488 nm/Em.535 nm) (QC tested).

Protocol

Biotinylated Human  ELISA

Report

CAR阳性表达率检测(Evaluation of CAR expression )

FACS Analysis of Anti-BCMA CAR Expression

CAR

293 cells were transfected with anti-BCMA-scFv and RFP tag. 2e5 of the cells were stained with B. FITC-labeled human BCMA / TNFRSF17 protein, Fc tag (Cat. No. BCA-HF254, 3 μg/ml) and C. FITC-labeled protein control. A. Non-transfected 293 cells and C. FITC-labeled protein control were used as negative control. RFP was used to evaluate CAR (anti-BCMA-scFv) expression and FITC was used to evaluate the binding activity of FITC-labeled human BCMA / TNFRSF17 protein, Fc tag (Cat. No. BCA-HF254) (QC tested).

Protocol

 

背景(Background)

Tumor necrosis factor receptor superfamily member 17 (TNFRSF17) is also known as B-cell maturation protein (BCMA), CD antigen CD269, which is a member of the TNF-receptor superfamily. TNFRSF17 contains one TNFR-Cys repeat. TNFRSF17 is expressed in mature B-cells, but not in T-cells or monocytes. TNFRSF17 is receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. TNFRSF17 promotes B-cell survival and plays a role in the regulation of humoral immunity. TNFRSF17 can activate NF-kappa-B and JNK.

 

前沿进展

 
货号/价格

BCA-HF254-25ug / ¥3190

BCA-HF254-200ug / ¥19800

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BCMA靶点信息
英文全称:B-cell maturation protein
中文全称:B细胞成熟蛋白
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:44详情
最高研发阶段:批准上市
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