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Human Glypican 3 / GPC3 Protein, Fc Tag, low endotoxin

分子别名(Synonym)

GPC3,OCI5,Glypican-3,GTR2-2,MXR7,DGSX,SDYS ,SGB,SGBS,SGBS1

表达区间及表达系统(Source)

Human Glypican 3, Fc Tag, low endotoxin (GP3-H5258) is expressed from human 293 cells (HEK293). It contains AA Gln 25 - His 559 (Accession # P51654-1).

Predicted N-terminus: Gln 25 & Ser 359

蛋白结构(Molecular Characterization)

Online(Gln 25 - His 559) P51654-1

This protein carries a human IgG1 Fc tag at the C-terminus. This protein contains a furin-like convertase cleavage site, 355-RQYR-358, and will be partially processed into N and C-terminal fragment with calculated MW of 38.1 kDa and 49.3 kDa respectively. The protein migrates as 40 kDa, 60 kDa and 100-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human Glypican 3, Fc Tag, low endotoxin (Cat. No. GP3-H5258) SDS-PAGE gel

Human Glypican 3, Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human FGF basic, Tag Free (Cat. No. BFF-H4117) at 2 μg/mL (100 μL/well)can bind Human Glypican 3, Fc Tag (Cat. No. GP3-H5258) with a linear range of 4-63 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Report

Biotinylated Human ELISA

Immobilized Human Glypican 3, Fc Tag, low endotoxin (Cat. No. GP3-H5258) at 0.5 μg/mL (100 μL/well) can bind Anti-Human GPC3 Mab with a linear range of 0.8-6 ng/mL (Routinely tested).

Protocol

CAR阳性表达率检测(Evaluation of CAR expression )

FACS Analysis of Anti-GPC3 CAR Expression

CAR

293 cells were transfected with anti-GPC3-scFv and RFP tag. 2e5 of the cells were first stained with B. Human Glypican 3, Fc Tag, low endotoxin (Cat. No. GP3-H5258, 3 μg/ml) and C. human Fc tag protein control, followed by FITC-conjugated anti-human IgG Fc antibody. A. Non-transfected 293 cells and C. human Fc tag protein control were used as negative control. RFP was used to evaluate CAR (anti-GPC3-scFv) expression and FITC was used to evaluate the binding activity of Human Glypican 3, Fc Tag, low endotoxin (Cat. No. GP3-H5258).

Protocol

 

背景(Background)

Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3 / GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.

 

前沿进展

 
货号/价格

GP3-H5258-100ug / ¥3000

GP3-H5258-1mg (250ug X 4) / ¥19800

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