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Human SECTM1 Protein, His Tag

分子别名(Synonym)

SECTM1,K12

表达区间及表达系统(Source)

Human SECTM1, His Tag (SE1-H5227) is expressed from human 293 cells (HEK293). It contains AA Gln 29 - Gly 145 (Accession # AAH17716).

Predicted N-terminus: Gln 29

蛋白结构(Molecular Characterization)

Online(Gln 29 - Gly 145) AAH17716

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 13.5 kDa. The protein migrates as 18-22 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human SECTM1, His Tag (Cat. No. SE1-H5227) SDS-PAGE gel

Human SECTM1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human CD7, Llama IgG2b Fc Tag, low endotoxin (Cat. No. CD7-H5258) at 5 μg/mL (100 μL/well) can bind Human SECTM1, His Tag (Cat. No. SE1-H5227) with a linear range of 8-63 ng/mL (QC tested).

Protocol

背景(Background)

Secreted and transmembrane protein 1 (SECTM1) is also known as Protein K-12, which is belongs to the SECTM family, which is Inducted by IFNG/IFN-gamma (at protein level). SECTM1 is detected at the highest levels in peripheral blood leukocytes and breast cancer cell lines. SECTM1 may be involved in thymocyte signaling.

 

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