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 >  经MALS验证的蛋白>Integrin alpha V beta 3 >IT3-H52E3

Human Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein, His Tag&Tag Free (MALS verified)

分子别名(Synonym)

Integrin alpha V beta 3,ITGAV&ITGB3

表达区间及表达系统(Source)

Human ITGAV&ITGB3 Heterodimer Protein, His Tag&Tag Free (IT3-H52E3) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Val 992 (ITGAV) & Gly 27 - Asp 718 (ITGB3) (Accession # NP_002201.1(ITGAV) & NP_000203.2(ITGB3)).

Predicted N-terminus: Phe 31 (ITGAV) & Gly 27 (ITGB3)

Request for sequence

蛋白结构(Molecular Characterization)

Online(Phe 31 - Val 992 (ITGAV) & Gly 27 - Asp 718 (ITGB3)) NP_002201.1 (ITGAV) & NP_000203.2 (ITGB3)

Human ITGAV&ITGB3 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGAV and ITGB3, has a calculated MW of 112.9 kDa (ITGAV) and 81.8 kDa (ITGB3). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB3 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 120-145 kDa (ITGAV) and 85-95 kDa (ITGB3) respectively due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human ITGAV&ITGB3 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT3-H52E3) SDS-PAGE gel

Human ITGAV&ITGB3 Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Human ITGAV&ITGB3 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT3-H52E3) MALS images

The purity of Human ITGAV&ITGB3 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT3-H52E3) is more than 85% and the molecular weight of this protein is around 207-253 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human Fibronectin at 2 μg/mL (100 μL/well) can bind Human ITGAV&ITGB3 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT3-H52E3) with a linear range of 0.01-0.3 μg/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized Human Vitronectin at 2 μg/mL (100 μL/well) can bind Human ITGAV&ITGB3 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT3-H52E3) with a linear range of 10-156 ng/mL (Routinely tested).

Protocol

文献引用(Citations)

背景(Background)

Integrin alpha-V/beta-3 (ITGAV:ITGB3) is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Also, Integrin alpha-V/beta-3 acts as a receptor for herpes virus 8/HHV-8, coxsackievirus A9, Hantaan virus, cytomegalovirus/HHV-5, human metapneumovirus, human parechovirus 1 and west nile virus. Furthermore, in case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

 

前沿进展

 
 
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Integrin alpha V beta 3靶点信息
英文全称:Integrin alpha-V/beta-3
中文全称:整合素α-V/β-3复合体
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:14详情
最高研发阶段:临床三期
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