膜杰作
Star Staining
描述（Description）
The Human THRB (Luc) HEK293 Reporter Cell was engineered to not only express thyroid hormone response element (TRE), but also express the receptor full length human THRB (Gene ID: 7068), which can drive luciferase expressing systems by thyroid hormone (TH) or thyroid hormone receptor (THR) agonists stimulation. In the absence of TH or THR agonists, the THRB receptor is not activated and luminescence signal is low. In the presence of TH or THR agonists, the THRB pathway-activated luminescence can be detected in a dose-dependent manner.
应用说明（Application）
• Screen for THR-β-selective agonists.
生长特性（Growth Properties）
Adherent
筛选标记（Selection Marker）
Puromycin (2 μg/mL) + Hygromycin (20 μg/mL)
培养基（Culture Medium）
DMEM + 10% FBS
冻存液（Freeze Medium）
Serum-free cell cryopreservation medium
装量（Quantity）
1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
存储（Storage）
Frozen in liquid nitrogen.
支原体检测（Mycoplasma Testing）
Negative
无菌检测（Sterility Testing）
Negative
使用说明（Instructions for Use）
See data sheet for detailed culturing and assay protocol.
Application
Bioactivity analysis of THR-β-selective agonist (RLU).
The Human THRB (Luc) HEK293 Reporter Cell and Human THRA (Luc) HEK293 Reporter Cell (Cat.No.CHEK-ATF180) were incubated with serial dilutions of Resmetirom (a THR-β-selective agonist), respectively. The EC50 of Resmetirom determined on Human THRB (Luc) HEK293 Reporter Cell was approximately 4.4 μM.
Protocol
Bioactivity analysis of THR-β-selective agonist (FOLD).
The Human THRB (Luc) HEK293 Reporter Cell and Human THRA (Luc) HEK293 Reporter Cell (Cat.No.CHEK-ATF180) were incubated with serial dilutions of Resmetirom (a THR-β-selective agonist), respectively. The max induction fold of Resmetirom determined on Human THRB (Luc) HEK293 Reporter Cell was approximately 43.7, and on Human THRA (Luc) HEK293 Reporter Cell was approximately 2.2, which exhibited higher potency and selectivity for THR-β over THR-α.
Protocol
Signaling Bioassay
Response to thyroid hormone (RLU).
This reporter cell was incubated with serial dilutions of Liothyronine (a dual THR-α and THR-β agonist). The EC50 of Liothyronine was approximately 0.0044 μM.
Protocol
Response to thyroid hormone (FOLD).
This reporter cell was incubated with serial dilutions of Liothyronine (a dual THR-α and THR-β agonist). The max induction fold was approximately 142.44.
Protocol
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背景（Background）
Nuclear hormone receptor that can act as a repressor or activator of transcription. High affinity receptor for thyroid hormones, including triiodothyronine and thyroxine.
License Disclosure
This cell line is provided for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit modification of this cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license. Modifications of this cell line, transfer to another facility, or commercial use of the cell lines may require a separate license and additional fees. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.
