SARS-CoV-2 Spike RBD (XBB.1.5) ELISA Kit (For Vaccine Development)

背景（Background）

The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has posed a serious threat to human health. A rapid and effective assay kit detecting the levels of SARS-CoV-2 Spike RBD is urgently needed to accelerate the development of COVID-19 vaccines.

应用说明（Application）

This kit is developed for quantitative detection of SARS-CoV-2 Spike RBD (XBB.1.5) in samples.

It is for research use only.

重构方法（Reconstitution）

Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

存储（Storage）

1. Unopened kit should be stored at 2℃-8℃ upon receiving.

2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

原理（Assay Principles）

This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of SARS-CoV-2 Spike RBD (XBB.1.5). The kit consists of microplate pre-coated with Anti-SARS-CoV-2 Spike RBD Antibody (XBB.1.5), an SARS-CoV-2 Spike RBD (XBB.1.5)as Control,biotin-Anti-SARS-CoV-2 Spike RBD Antibody,HRP-Streptavidin and buffers.

Your experiment will include 6 simple steps:

a) Bring all reagents and samples to room temperature (20℃-25℃) before use.

b) Add your sample to the plate, take the SARS-CoV-2 Spike RBD as Control sample. The samples and Control sample are diluted by Dilution Buffer.

c) Add the Secondary antibody biotin-Anti-SARS-CoV-2 Spike RBD Antibody diluted by Dilution Buffer to the plate.

d) Add the diluted Streptavidin-HRP to the plate.

e) Wash the plate and add TMB or other colorimetric HRP substrate.

f) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.