Human Anti-RSV-F0 Antibody IgG Titer ELISA Assay Kit (Pre-Fusion glycoprotein F0)

背景（Background）

Respiratory syncytial virus (RSV) is a highly contagious virus causing severe infection in infants and the elderly. Various approaches are being used to develop an effective RSV vaccine. The RSV fusion (F) subunit, particularly the cleaved trimeric pre-fusion F, is one of the most promising vaccine candidates under development.

To facilitate the RSV-related research, drug trials and vaccine development, a high-throughput assay to measure IgG antibodies against the virus is in urgent need.

应用说明（Application）

The kit is developed for titer measurement of Anti-RSV-F0 Antibody IgG (Pre-Fusion glycoprotein F0) in Human serum.

It is for research use only.

存储（Storage）

1. Unopened kit should be stored at 2℃-8℃ upon receiving.

2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

原理（Assay Principles）

This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Anti-RSV-F0 antibodies in Human serum by Pre-Fusion glycoprotein F0 (RSV). The Kit consists of Pre-coated Pre-Fusion glycoprotein F0 (RSV) Microplate, Positive Control, Negative Control and HRP-conjugated antibody.

Your experiment will include 4 simple steps:

a) The samples and Control are diluted by Dilution Buffer.Add your sample to the plate.

b) Add the HRP-conjugated antibody diluted by Dliution Buffer to the plate.

c) Wash the plate and add TMB or other colorimetric HRP substrate.

d) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.