膜杰作
Star Staining
描述（Description）
The CHO/ Cynomolgus Glypican-3 (GPC3) Stable Cell Line was engineered to express the receptor full length Cynomolgus Glypican-3 (Gene ID:9541). Surface expression of Cynomolgus Glypican-3 was confirmed by flow cytometry.
应用说明（Application）
• Useful for cell-based Glypican-3 binding assay
生长特性（Growth Properties）
Adherent
筛选标记（Selection Marker）
Hygromycin (20 μg/mL)
培养基（Culture Medium）
F-12K + 10% FBS
冻存液（Freeze Medium）
Serum-free cell cryopreservation medium
装量（Quantity）
1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
存储（Storage）
Frozen in liquid nitrogen.
支原体检测（Mycoplasma Testing）
Negative
无菌检测（Sterility Testing）
Negative
使用说明（Instructions for Use）
See data sheet for detailed culturing and assay protocol.
Receptor Assay
Expression analysis of Cynomolgus Glypican-3 (GPC3) on CHO/Cynomolgus Glypican-3 (GPC3) Stable Cell Line by FACS.
Cell surface staining was performed on CHO/Cynomolgus Glypican-3 (GPC3) Stable Cell Line or negative control cell using anti-Cynomolgus Glypican-3 (GPC3) Antibody followed by staining with PE anti-human IgG Fc Antibody.
Protocol
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背景（Background）
Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3 / GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.
License Disclosure
This cell line is provided for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit modification of this cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license. Modifications of this cell line, transfer to another facility, or commercial use of the cell lines may require a separate license and additional fees. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.
