膜杰作
Star Staining
表达区间及表达系统（Source）
Varicella zoster virus (strain Oka vaccine) gI&gE Protein, His Tag&Twin-Strep Tag (GIE-V5283) is expressed from human 293 cells (HEK293). It contains AA Leu 21-Leu 274 & Ser 31 - Ala 546 (Accession # Q77NN4 & Q9J3M8).
Predicted N-terminus: Leu 21 & Ser 31
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag and a twin strep tag at the C-terminus. The protein has a calculated MW of 30.1 kDa & 61.7 kDa. The protein migrates as 35-42 kDa and 60-90 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Varicella zoster virus (strain Oka vaccine) gI&gE Protein, His Tag&Twin-Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-HPLC
The purity of Varicella zoster virus (strain Oka vaccine) gI&gE Protein, His Tag&Twin-Strep Tag (Cat. No. GIE-V5283) was greater than 85% as determined by SEC-HPLC.
活性（Bioactivity）-ELISA
Immobilized Varicella zoster virus (strain Oka vaccine) gI&gE Protein, His Tag&Twin-Strep Tag (Cat. No. GIE-V5283) at 1 μg/mL (100 μL/well) can bind Anti-Varicella-Zoster Virus Antibody with a linear range of 0.1-1 ng/mL (QC tested).
Protocol
背景（Background）
Varicella-zoster virus (VZV) glycoprotein gI is a type 1 transmembrane glycoprotein which is one component of the heterodimeric gE:gI Fc receptor complex. Like VZV gE, VZV gI was phosphorylated in both VZV-infected cells and gI-transfected cells.
