膜杰作
Star Staining
抗体来源（Source）
Monoclonal Anti-PEG Antibody, Mouse IgG1 (5G3-1C6) is a chimeric monoclonal antibody recombinantly expressed from human 293 cells (HEK293), which combines the variable region of a mouse monoclonal antibody with mouse IgG1 constant domain. The mouse monoclonal antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with PEG.
亚型（Isotype）
Mouse IgG1 | Mouse Kappa
特异性（Specificity）
This product is a specific antibody specifically reacts with PEG.
应用（Application）
ELISA
纯度（Purity）
>90% as determined by SDS-PAGE.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20 to -70°C for 12 months in lyophilized state from date of receipt;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Monoclonal Anti-PEG Antibody, Mouse IgG1 (5G3-1C6) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized PEG Coated Plates, Clear, 12×8-Well Strips, White Frame at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-PEG Antibody, Mouse IgG1 (5G3-1C6) (Cat. No. PEG-M687) with a linear range of 5-156 ng/mL (QC tested).
Protocol
Immobilized 5KD-PEG/20KD-PEG/40KD-PEG at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-PEG Antibody, Mouse IgG1 (5G3-1C6) (Cat. No. PEG-M687) with a linear range of 5-78 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-MSD
MSD assay for the Detection of anti-IFN-PEG(Hide drug info) in Human Serum.
Immobilized Biotin Human IFN on MSD GoldTM 96-well Streptavidin Plate,can bind anti-IFN-PEG when detected by Sulfo Anti-PEG Antibody.Use the MESO QuickPlex SQ 120 MM instrument for analysis.
Protocol
背景（Background）
Polyethylene glycol, referred to as PEG, is used as an inactive ingredient in the pharmaceutical industry as a solvent, plasticizer, surfactant, ointments, and suppository base, and tablet and capsule lubricant. PEG has low toxicity with systemic absorption less than 0.5%.
PEGylation occurs when PEGs are attached to various protein medications, allowing for greater solubility for certain drugs. Examples of PEGylated medications include PEG-interferon alpha (Pegintron) and PEG-filgrastim (Neulasta)
