膜杰作
Star Staining
描述（Description）
The HEK293/Human ASGR1&ASGR2 Stable Cell Line was engineered to express the receptors full length human ASGR1 (Gene ID: 432) and ASGR2 (Gene ID: 433). Surface expression of human ASGR1 and ASGR2 was confirmed by flow cytometry.
应用说明（Application）
• Useful for cell-based ASGR1 or ASGR2 binding assay
生长特性（Growth Properties）
Adherent
筛选标记（Selection Marker）
Puromycin (2 μg/mL) + Hygromycin (10 μg/mL)
培养基（Culture Medium）
DMEM + 10% FBS
冻存液（Freeze Medium）
Serum-free cell cryopreservation medium
装量（Quantity）
1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
存储（Storage）
Frozen in liquid nitrogen.
支原体检测（Mycoplasma Testing）
Negative
无菌检测（Sterility Testing）
Negative
使用说明（Instructions for Use）
See data sheet for detailed culturing and assay protocol.
活性（Bioactivity）
Co-expression analysis of human ASGR1 and ASGR2 on HEK293/Human ASGR1&ASGR2 Stable Cell Line by FACS.
Cell surface staining was performed on HEK293/Human ASGR1&ASGR2 Stable Cell Line or negative control cell using APC-labeled anti-ASGR1 antibody and PE-labeled anti-ASGR2 antibody.
Protocol
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背景（Background）
ASGPR, a transmembrane C-type lectin, recognizes a wide variety of ligands that contain either terminal galactose (Gal) or N-acetylgalactosamine (GalNAc) residues and has been identified as a highly selective receptor on hepatocytes. The ASGR is composed of both a major (ASGR1) and minor subunit (ASGR2).
License Disclosure
This cell line is provided for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit modification of this cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license. Modifications of this cell line, transfer to another facility, or commercial use of the cell lines may require a separate license and additional fees. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.
