登录 | 注册    关注公众号  
微信公众号
搜索
 >  Protein>Glypican 1 >GP1-H82E3

Biotinylated Human Glypican 1 Protein, His,Avitag™ (MALS verified)

分子别名(Synonym)

Glypican 1,GPC1

表达区间及表达系统(Source)

Biotinylated Human Glypican 1 Protein, His,Avitag (GP1-H82E3) is expressed from human 293 cells (HEK293). It contains AA Asp 24 - Ser 530 (Accession # P35052-1).

Predicted N-terminus: Asp 24

Request for sequence

蛋白结构(Molecular Characterization)

Glypican 1 Structure

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 59.7 kDa. The protein migrates as 65-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Glypican 1 SDS-PAGE

Biotinylated Human Glypican 1 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS

Glypican 1 SEC-MALS

The purity of Biotinylated Human Glypican 1 Protein, His,Avitag (Cat. No. GP1-H82E3) is more than 90% and the molecular weight of this protein is around 55-75 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Glypican 1 ELISA

Immobilized Human FGF basic, premium grade (Cat. No. BFF-H4117) at 2 μg/mL (100 μL/well) can bind Biotinylated Human Glypican 1 Protein, His,Avitag (Cat. No. GP1-H82E3) with a linear range of 2-40 ng/mL (QC tested).

Protocol

Glypican 1 ELISA

Immobilized Human FGF basic (154aa) Protein, premium grade (Cat. No. BFF-H5115) at 2 μg/mL (100 μL/well) can bind Biotinylated Human Glypican 1 Protein, His,Avitag (Cat. No. GP1-H82E3) with a linear range of 2-40 ng/mL (Routinely tested).

Protocol

 

背景(Background)

Glypican 1 has been shown to interact with SLIT2. This protein is involved in the misfolding of normal prion proteins in the cell membrane to the infectious prion form. Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.

 

前沿进展

 
 
货号/价格
产品推荐
文档
联系电话:
+86 400-682-2521(全国)
010-53681107(北京)
021-50850665(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
tech.cn@acrobiosystems.com
Glypican 1靶点信息
英文全称:Glypican-1
中文全称:磷脂酰肌醇聚糖1
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:1详情
最高研发阶段:临床一期
查看更多信息
前沿进展
点击查看详细

消息提示

请输入您的联系方式,再点击提交!

确定