膜杰作
Star Staining
产品描述（Product Details）
| Assay Type | Sandwich-ELISA |
| Analyte | IL-17A/CTLA8 |
| Format | 96T |
| Reactivity | Human |
| Sensitivity | <6pg/mL |
| Standard Curve Range | 31.25 pg/mL-2000 pg/ml |
| Assay Time | 2hr |
| Suitable Sample Type | For the quantitative determination of human IL-17A/CTLA8 in Cell Culture Supernatants, Plasma, Serum. |
| Sample volume | 50 μL |
组分（Materials Provided）
| ID | Components | Size |
| CEA092-C01 | Pre-coated Anti-IL-17A Antibody Microplate | 1 plate |
| CEA092-C02 | Human IL-17A Standard | 20μg ×2 |
| CEA092-C03 | Biotin-Anti-IL-17A Antibody Con. Solution | 300 μL |
| CEA092-C04 | Biotin-Antibody Dilution Buffer | 8 mL |
| CEA092-C05 | Streptavidin-HRP Con. Solution | 500 uL |
| CEA092-C06 | Streptavidin-HRP Dilution Buffer | 15 mL |
| CEA092-C07 | 20× Washing Buffer | 50 mL |
| CEA092-C08 | Sample Dilution Buffer | 15 mL ×2 |
| CEA092-C09 | Substrate Solution | 12 mL |
| CEA092-C10 | Stop Solution | 6 mL |
背景（Background）
ClinMax™ ELISA Kit is convenient ready-to-use immunoassay Kit, specifically designed to quantitate human IL-17A/CTLA8 that is present in complex biological samples, such as human serum, plasma, and cell culture supernates.
A comprehensive validation of the ELISA method was performed following the ICH M10 on bioanalytical method validation and the FDA’s bioanalytical method validation guidance for industry. This validation included assessments of linearity, accuracy, precision, dilution linearity, recovery, and the hook effect. For details information, please refer to the DS.
ClinMax™ ELISA Kits are manufactured in a GMP-certified facility and comply to the ISO 13485 standard, ensuring a high level of quality and reliability.
应用说明（Application）
The kit is developed for quantitative detection of human IL-17A/CTLA8 in serum, plasma and cell culture supernates.
It is for research use only.
存储（Storage）
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
原理（Assay Principles）
典型数据-Typical Data Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of IL-17A is less than 6 pg/mL.
验证（Validation）
批内差异（Intra-Assay Statistics）
Ten replicates of each of 4 samples containing different IL-17A concentrations were tested in one assay. Acceptable criteria: CV＜10%.
批间差异（Inter-Assay Statistics）
Five samples containing different concentrations of IL-17A were tested in independent assays. Acceptable criteria: CV<15%.
回收率（Recovery）
Recombinant IL-17A was spiked into 3 human serum samples, and then analyzed. The average recovery of IL-17A for serum samples is 85.37%.
背景（Background）：IL-17A
Interleukin-17A is a protein that in humans is encoded by the IL17A gene. In rodents, IL-17A used to be referred to as CTLA8, after the similarity with a viral gene.The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO).
