膜杰作
Star Staining
描述（Description）
The Human IL-2 R beta/IL-2 R gamma (Luc) HEK293 Reporter Cell was engineered to not only express STAT5 signaling response element, but also express the receptors full length human IL-2 R beta (Gene ID: 3560) and IL-2 R gamma (Gene ID: 3561). When stimulated with human IL-2 protein, receptor-mediate signaling drives STAT5-mediated luminescence.
应用说明（Application）
• Bioactivity detection of human IL-2 fusion protein
• Bioactivity detection of human IL-15 fusion protein
生长特性（Growth Properties）
Adherent
筛选标记（Selection Marker）
Puromycin (2 μg/mL) + Zeocin (20 μg/mL) + G418 (200 μg/mL)
培养基（Culture Medium）
DMEM + 10% FBS
冻存液（Freeze Medium）
Serum-free cell cryopreservation medium
装量（Quantity）
1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
存储（Storage）
Frozen in liquid nitrogen.
支原体检测（Mycoplasma Testing）
Negative
无菌检测（Sterility Testing）
Negative
使用说明（Instructions for Use）
See data sheet for detailed culturing and assay protocol.
Receptor Assay
Co-expression analysis of human IL-2 R beta and IL-2 R gamma on Human IL-2 R beta/IL-2 R gamma (Luc) HEK293 Reporter Cell by FACS.
Cell surface staining was performed on Human IL-2 R beta/IL-2 R gamma (Luc) HEK293 Reporter Cell or negative control cell using PE-labeled anti-IL-2 R beta antibody and APC-labeled anti-IL-2 R gamma antibody.
Protocol
Signaling Bioassay
Response to human IL-2 protein (RLU).
The Human IL-2 R beta/IL-2 R gamma (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human IL-2 protein (Cat.No.IL2-H5215). The EC50 was approximately 0.0025 μg/mL.
Protocol
Response to human IL-2 protein (FOLD).
The Human IL-2 R beta/IL-2 R gamma (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human IL-2 protein (Cat.No.IL2-H5215). The max induction fold was approximately 99.74.
Protocol
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背景（Background）
Both Interleukin-2 receptor subunit beta and Interleukin-2 receptor subunit gamma are receptor for interleukin-2. Common subunit for the receptors for a variety of interleukins. Interacts with SHB upon interleukin stimulation. Probably in association with IL15RA, involved in the stimulation of neutrophil phagocytosis by IL15. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit.
License Disclosure
This cell line is provided for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit modification of this cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license. Modifications of this cell line, transfer to another facility, or commercial use of the cell lines may require a separate license and additional fees. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.
