膜杰作
Star Staining
| ID | Components | Size |
| RES050-C01 | Pre-coated Anti-HIV-1 p24 Antibody Microplate | 1 plate |
| RES050-C02 | HIV-1 p24 Standard | 20 μg |
| RES050-C03 | Biotin-Anti-HIV-1 p24 Antibody | 20 μg |
| RES050-C04 | Streptavidin-HRP | 50 μL |
| RES050-C05 | 10xWashing Buffer | 50 mL |
| RES050-C06 | 2xDilution Buffer | 50 mL |
| RES050-C07 | Substrate Solution | 12 mL |
| RES050-C08 | Stop Solution | 7 mL |
背景（Background）
HIV-1 p24 ELISA Kit is based on ELISA sandwich method and designed to measure HIV-1 p24 levels in samples from CAR-T product preparation processing. It contains recombinant HIV-1 p24 and a pair of antibodies against the recombinant factor, which are provided by ACROBiosystems. Results are obtained by four parameter logistic curve that were parallel to the standard curves obtained. The verification results indicate that this kit can be used for the quantitative determination of HIV-1 p24 concentrations.
应用说明（Application）
This kit is developed for quantitative detection of Human Immunodeficiency Virus-1 p24 (HIV-1 p24) in samples from CAR-T product preparation processing where it is used as a lentivirus vector.
It is for research use only.
存储（Storage）
Unopened kit should be stored at 2°C -8°C upon receiving.
Find the expiration date on the outside packaging and do not use reagents past their expiration date.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
背景（Background）：Capsid protein p24
Human Immunodeficiency Virus (HIV) can be divided into two major types, HIV type 1 (HIV-1) and HIV type 2 (HIV-2). HIV-1 is related to viruses found in chimpanzees and gorillas living in western Africa. The gag gene of HIV-1 encodes a precursor protein known as Pr55Gag. The viral protease PR cleaves this precursor to generate p17, p24, p7, and p6 proteins which are required for virus particle assembly. p24 is a major viral core structural protein, which is the main component of HIV virus and plays an important role in the process of virus reproduction. Usually, p24 protein can be detected in blood 2-3 weeks after hiv infection.Its measurement is commonly used as an indicator of HIV-1 infection and viral load. Therefore, it's helpful to develop the Human Immunodeficiency Virus type 1 (HIV-1) p24 ELISA Kit to quantitative detection the HIV-1 p24 antigen in cell culture supernates .
