膜杰作
Star Staining
表达区间及表达系统（Source）
PE-Labeled Human HLA-A*11:01&B2M&EBV LMP2 (SSCSSCPLSK) Tetramer Protein (HL2-HP2H5) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Thr 305 (HLA-A*11:01) & Ile 21 - Met 119 (B2M) & SSCSSCPLSK peptide (Accession # Q5S3G3-1 (HLA-A*11:01) & P61769 (B2M) & SSCSSCPLSK).
Predicted N-terminus: Gly 25 & Ile 21
Request for sequence
蛋白结构（Molecular Characterization）
PE-Labeled Human HLA-A*11:01&B2M&EBV LMP2 (SSCSSCPLSK) Tetramer Protein is assembled by biotinylated monomer and PE-labeled streptavidin.
Biotinylated Human HLA-A*11:01&B2M&EBV LMP2 (SSCSSCPLSK) Complex Protein is produced by co-expression of HLA and B2M loaded with EBV LMP2 peptide. Biotinylated Human HLA-A*11:01&B2M&EBV LMP2 (SSCSSCPLSK) Complex Protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
偶联（Conjugate）
PE
Excitation Wavelength: 488 nm / 561 nm
Emission Wavelength: 575 nm
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, 1% BSA, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
活性（Bioactivity）-ELISA
Immobilized PE-Labeled Human HLA-A*11:01&B2M&EBV LMP2 (SSCSSCPLSK) Tetramer Protein (Cat. No. HL2-HP2H5) at 1 μg/mL (100 μL/well) can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
背景（Background）
Epstein-Bar Virus (EBV), also known as human herpesvirus 4, belongs to gamma herpes virus family and is a very common human virus worldwide. EBV causes infectious mononucleosis (IM) and also associates to some specific types of cancers such as Burkitt’s lymphoma (BL) and gastric carcinoma (GC). Glycoprotein B (gB) plays an important role in viral entry by binding with αvβ6/αvβ8 integrins to trigger the membrane fusion and entry process of epithelial cells, which makes it become an great target for EBV research. Epstein-Bar Virus (EBV), also known as human herpesvirus 4, belongs to gamma herpes virus family and is a very common human virus worldwide. EBV causes infectious mononucleosis (IM) and also associates to some specific types of cancers such as Burkitt’s lymphoma (BL) and gastric carcinoma (GC). Glycoprotein B (gB) plays an important role in viral entry by binding with αvβ6/αvβ8 integrins to trigger the membrane fusion and entry process of epithelial cells, which makes it become an great target for EBV research. The Human HLA-A*1101 EBV LMP2 (SSCSSCPLSK)complex protein is a complex of HLA-A*1101 of the MHC Class I, B2M and SSCSSCPLSK peptide of the EBV LMP2.
