膜杰作
Star Staining
表达区间及表达系统（Source）
Human IL-4 R alpha & IL-13 R alpha 1 Protein, Fc Tag&Fc Tag (ILR-H525b) is expressed from human 293 cells (HEK293). It contains AA Met 26 - His 232 & Gly 22 - Thr 343 (Accession # P24394-1 & NP_001551.1).
Predicted N-terminus: Met 26 & Gly 22
Request for sequence
蛋白结构（Molecular Characterization）
Human IL-4 R alpha & IL-13 R alpha 1 Protein, Fc Tag&Fc Tag is produced by co-expression of IL-4 R alpha and IL-13 R alpha 1, has a calculated MW of 50.2 kDa & 63.1 kDa. The protein migrates as 65-95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human IL-4 R alpha & IL-13 R alpha 1 Protein, Fc Tag&Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human IL-4 R alpha & IL-13 R alpha 1 Protein, Fc Tag&Fc Tag (Cat. No. ILR-H525b) is more than 90% and the molecular weight of this protein is around 140-170 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human IL-4 R alpha & IL-13 R alpha 1 Protein, Fc Tag&Fc Tag (Cat. No. ILR-H525b) at 5 μg/mL (100 μL/well) can bind Human IL-4, His Tag (Cat. No. IL4-H52H9) with a linear range of 0.6-5 ng/mL (QC tested).
Protocol
Immobilized Human IL-13 Protein, His Tag, premium grade (Cat. No. IL3-H52H4) at 5 μg/mL (100 μL/well) can bind Human IL-4 R alpha & IL-13 R alpha 1 Protein, Fc Tag&Fc Tag (Cat. No. ILR-H525b) with a linear range of 0.02-0.625 μg/mL (Routinely tested).
Protocol
背景（Background）
Interleukin 4 Receptor (IL4R) also known as CD124, IL4Rα and BSF receptor, is a type I cytokine receptor produced by activated Th2 cells and mast cells, and plays an important role in Th2-biased immune responses, alternative macrophage activation, mucosal immunity, allergic inflammation, tumor progression, and atherogenesis. A soluble form of the encoded IL4R protein can be produced by an alternate splice variant or by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. IL4R can alternatively associate with IL13Ra1 to form the type II receptor which is responsive to both IL4 and IL13. Interleukin-4 receptor has been shown to interact with SHC1.
