膜杰作
Star Staining
描述（Description）
The ISRE (Luc) HEK293 Reporter Cell was engineered to express IFN-stimulated response elements (ISRE) driving luciferase expressing systems. When stimulated with human Type I interferons (IFNs), receptor-mediated signaling can drive IFN-stimulated gene factor 3 (ISGF3)-mediated luminescence.
应用说明（Application）
• Screen for activators or inhibitors of Type I interferons.
生长特性（Growth Properties）
Adherent
筛选标记（Selection Marker）
Puromycin (2 μg/mL)
培养基（Culture Medium）
DMEM + 10% FBS
冻存液（Freeze Medium）
Serum-free cell cryopreservation medium
装量（Quantity）
1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
存储（Storage）
Frozen in liquid nitrogen.
支原体检测（Mycoplasma Testing）
Negative
无菌检测（Sterility Testing）
Negative
使用说明（Instructions for Use）
See data sheet for detailed culturing and assay protocol.
Signaling Bioassay
Response to human IFN-alpha 2b protein (RLU).
The ISRE (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human IFN-alpha 2b protein (Cat.No.IFB-H5253). The EC50 was approximately 0.026 μg/mL.
Protocol
Response to human IFN-alpha 2b protein (FOLD).
The ISRE (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human IFN-alpha 2b protein (Cat.No.IFB-H5253). The max induction fold was approximately 89.
Protocol
如有相关细胞池需求请联系我们
背景（Background）
ISRE is a marker of interferon stimulated genes (ISGs), which mainly include enzymes that can inhibit virus replication. Type I (such as IFN α and IFN β） and type III interferon (such as IFN-λ) can activate STAT1 and STAT2, and the activated STAT1, STAT2, and IRF9 form a protein complex called ISGF3. The ISGF3 protein complex binds to the promoter containing the ISRE element (interferon-stimulated response element) and activates the expression of related genes.
License Disclosure
This cell line is provided for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit modification of this cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license. Modifications of this cell line, transfer to another facility, or commercial use of the cell lines may require a separate license and additional fees. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.
