膜杰作
Star Staining
抗体来源（Source）
Monoclonal Anti-PTX Antibody, Mouse IgG2a is recombinantly produced from human 293 cells (HEK293).
亚型（Isotype）
Mouse IgG2a/kappa
特异性（Specificity）
This product is a specific antibody specifically reacts with PTX.
应用（Application）
ELISA
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Monoclonal Anti-PTX Antibody, Mouse IgG2a on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Monoclonal Anti-PTX Antibody, Mouse IgG2a (Cat. No. PTX-S343) is more than 90% and the molecular weight of this protein is around 140-160 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized ADC-PTX at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-PTX Antibody, Mouse IgG2a (Cat. No. PTX-S343) with a linear range of 0.106-6.767 ng/mL (QC tested).
Protocol
Immobilized Human TROP-2 / TACSTD2 Protein, His Tag (MALS verified) (Cat. No. TR2-H5223) at 2 μg/mL, add increasing concentrations of ADC-PTX, and then add Monoclonal Anti-PTX Antibody, Mouse IgG2a (Cat. No. PTX-S343) at 0.54 μg/mL. Detection was performed using HRP-conjugated Goat Anti-Mouse IgG with sensitivity of 0.04 ng/mL (Routinely tested).
Protocol
背景（Background）
Paclitaxel(ADC-PTX), a natural anticancer drug, has been widely used in the treatment of breast cancer, ovarian cancer and some head, neck and lung cancers. As a diterpenoid alkaloid with anticancer activity, paclitaxel has been greatly favored by botanists, chemists, pharmacologists and molecular biologists due to its novel and complex chemical structure, extensive and significant biological activity, new and unique mechanism of action, and scarce natural resources, making it the star and research focus of anticancer in the second half of the 20th century.
