膜杰作
Star Staining
产品描述（Product Details）
| Assay Type | Sandwich-ELISA |
| Analyte | Pyrophosphatase |
| Format | 96T |
| Reactivity | Human |
| Regulatory Status | RUO |
| Sensitivity | < 9.38 pg/mL |
| Standard Curve Range | 9.375 pg/mL-300 pg/mL |
| Assay Time | 3 hr 20 min |
| Suitable Sample Type | For the quantitative determination of Pyrophosphatase in Cell Culture Supernatants. |
| Sample volume | 100 μL |
组分（Materials Provided）
| ID | Components | Size |
| RES005-C01 | Pre-coated Anti-Pyrophosphatase Antibody Microplate | 1 plate |
| RES005-C02 | Pyrophosphatase Standard | 100 μL |
| RES005-C03 | Biotin-Anti-Pyrophosphatase Antibody | 15 μg |
| RES005-C04 | Streptavidin-HRP | 50 μL |
| RES005-C05 | 20xWashing Buffer | 50 mL |
| RES005-C06 | 2xDilution Buffer | 50 mL |
| RES005-C07 | Substrate Solution | 12 mL |
| RES005-C08 | Stop Solution | 7 mL |
背景（Background）
Pyrophosphatase ELISA Kit(Residue Testing) is based on ELISA sandwich method and designed to detect and quantitative determine of Pyrophosphatase in mRNA preparation processing. It contains Pyrophosphatase and a pair of antibodies against the recombinant enzyme, which are provided by ACROBiosystems. Results are obtained by four parameter logistic curve that were parallel to the standard curves obtained. The verification results indicate that this kit can be used for the quantitative determination of Pyrophosphatase (ACROBiosystems, cat#PYE-T5143) concentrations. The specificity has been verified.
应用说明（Application）
Pyrophosphatase ELISA Kit(Residue Testing) was developed for the detection and quantitative determination of Pyrophosphatase in mRNA preparation processing where Pyrophosphatase is used as an row agent.
It is for research use only.
重构方法（Reconstitution）
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储（Storage）
The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
原理（Assay Principles）
This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of Pyrophosphatase. The kit consists of Pre-coated Anti-Pyrophosphatase Antibody Microplate and Pyrophosphatase Standard and Biotin-Anti-Pyrophosphatase Antibody and Streptavidin-HRP and buffers.
Your experiment will include 6 simple steps:
a) Bring all reagents to room temperature(20℃-25℃) before use.
b) Add your sample to the plate and take the Pyrophosphatase as standard. The samples and standard are diluted by Dilution Buffer.
c) Add the Biotin-Anti-Pyrophosphatase Antibody diluted by Dilution Buffer to the plate.
d) Wash the plate and add the Streptavidin-HRP diluted by Dilution Buffer to the plate.
e) Wash the plate and add TMB.
f) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated by the absorbance at 450 nm minus the absorbance at 630 nm to remove background disturbance before statistical analysis. The OD Value reflects the amount of bound enzyme.
典型数据-Typical Data Please refer to Ds document for the assay protocol.
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
验证（Validation）
批内差异（Intra-Assay Statistics）
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision, Intra-Assay Precision CV＜15%.
批间差异（Inter-Assay Statistics）
Three samples of known concentration were tested in three separate assays to assess inter-assay precision, Inter-Assay Precision CV<15%.
回收率（Recovery）
Three Pyrophosphatase with different concentrations were tested to calculate the recovery rate.
背景（Background）：Pyrophosphatase
Pyrophosphatase catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate, it can hydrolyze inorganic pyrophosphate generated with the reaction to avoid its inhibition of the reaction system. Pyrophosphatase is usually used to increase RNA yield in reverse transcription reactions. As a key raw material for in vitro transcription (IVT) of RNA, Pyrophosphatase needs to detect the residues as a protein component. This ELISA kit can be used to detect the residue of Pyrophosphatase in mRNA stock solution.
