膜杰作
Star Staining
分子别名（Synonym）
CD7,GP40,TP41,LEU-9,Tp40
表达区间及表达系统（Source）
Cynomolgus CD7 Protein, His Tag (CD7-C52H7) is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Ser 177 (Accession # A0A2K5VA18).
Predicted N-terminus: Ala 26
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 17.9 kDa. The protein migrates as 27-40 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Cynomolgus CD7 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Cynomolgus CD7 Protein, His Tag (Cat. No. CD7-C52H7) is more than 90% and the molecular weight of this protein is around 40-50 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-SPR
Cynomolgus CD7 Protein, His Tag (Cat. No. CD7-C52H7) immobilized on CM5 Chip can bind Human SECTM1, His Tag (Cat. No. SE1-H5227) with an affinity constant of 24.7 nM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
背景（Background）
T-cell antigen CD7 (CD7) is also known as GP40, LEU-9, TP41 and Tp40. CD7 is a protein that in humans is encoded by the CD7 gene, this gene encodes a transmembrane protein which is a member of the immunoglobulin superfamily. CD7 has been shown to interact with PIK3R1. This protein is found on thymocytes and mature T cells. It plays an essential role in T-cell interactions and also in T-cell/B-cell interaction during early lymphoid development.
