膜杰作
Star Staining
描述（Description）
The HEK293/Human NAPI-IIb Stable Cell Line was engineered to express the receptor full length human NAPI-IIb (Gene ID: 10568), used to mimic cancer target cells. Surface expression of Human NAPI-IIb was confirmed by flow cytometry.
应用说明（Application）
• Useful for cell-based NAPI-IIb binding assay
生长特性（Growth Properties）
Adherent
筛选标记（Selection Marker）
Puromycin (2 μg/mL)
培养基（Culture Medium）
DMEM + 10% FBS
冻存液（Freeze Medium）
Serum-free cell cryopreservation medium
装量（Quantity）
1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
存储（Storage）
Frozen in liquid nitrogen.
支原体检测（Mycoplasma Testing）
Negative
无菌检测（Sterility Testing）
Negative
使用说明（Instructions for Use）
See data sheet for detailed culturing and assay protocol.
活性（Bioactivity）
Expression analysis of human NAPI-IIb on HEK293/Human NAPI-IIb Stable Cell Line by FACS.
HEK293/Human NAPI-IIb Stable Cell Line or negative control cell was fixed with 4% paraformaldehyde followed by staining with PE-labeled anti-human NAPI-IIb antibody.
Protocol
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背景（Background）
NAPI-IIb, NaPi-2b, Npt2b, or SLC34A2 enables sodium:phosphate symporter activity. Acts upstream of or within in utero embryonic development and phosphate ion transport. Located in apical plasma membrane and brush border. Is expressed in several structures, including egg cylinder; genitourinary system; small intestine; and tooth. Human ortholog(s) of this gene implicated in pulmonary alveolar microlithiasis. Orthologous to human SLC34A2 (solute carrier family 34 member 2). [provided by Alliance of Genome Resources, Apr 2022]
License Disclosure
This cell line is provided for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit modification of this cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license. Modifications of this cell line, transfer to another facility, or commercial use of the cell lines may require a separate license and additional fees. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.
