膜杰作
Star Staining
描述（Description）
The Human 5-HT1A (Luc) HEK293 Reporter Cell was engineered to not only express CREB signaling response element, but also express the receptor full length human 5-HT1A (Gene ID: 3350) and mGFP by a viral P2A self-cleaving peptide. When stimulated with human 5-HT1A agonist, the agonist/5-HT1A interaction drives CREB-mediated luminescence. Inhibition of activated 5-HT1A signaling by corresponding inhibitors results in a decrease in luminescence.
应用说明（Application）
Screening for 5-HT1A receptor agonists and antagonists.
生长特性（Growth Properties）
Adherent
筛选标记（Selection Marker）
Puromycin (2 μg/mL) + Hygromycin (20 μg/mL)
培养基（Culture Medium）
DMEM + 10% FBS
冻存液（Freeze Medium）
Serum-free cell cryopreservation medium
装量（Quantity）
1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
存储（Storage）
Frozen in liquid nitrogen.
使用说明（Instructions for Use）
See data sheet for detailed culturing and assay protocol.
背景（Background）
This gene encodes a G protein-coupled receptor for 5-hydroxytryptamine (serotonin), and belongs to the 5-hydroxytryptamine receptor subfamily. Serotonin has been implicated in a number of physiologic processes and pathologic conditions. Inactivation of this gene in mice results in behavior consistent with an increased anxiety and stress response. Mutation in the promoter of this gene has been associated with menstrual cycle-dependent periodic fevers.
License Disclosure
This cell line is provided for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit modification of this cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license. Modifications of this cell line, transfer to another facility, or commercial use of the cell lines may require a separate license and additional fees. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.
