膜杰作
Star Staining
表达区间及表达系统（Source）
Japanese encephalitis virus (strain SA-14) Envelope protein E, His Tag (MALS verified) (ENE-J52H5) is expressed from human 293 cells (HEK293). It contains AA Phe 295-Ala 700 (Accession # P27395-1).
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 64.6 kDa. The protein migrates as 48-53 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Japanese encephalitis virus (strain SA-14) Envelope protein E, His Tag (MALS verified) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Japanese encephalitis virus (strain SA-14) Envelope protein E, His Tag (MALS verified) (Cat. No. ENE-J52H5) is more than 85% and the molecular weight of this protein is around 40-55 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Japanese encephalitis virus (strain SA-14) Envelope protein E, His Tag (MALS verified) (Cat. No. ENE-J52H5) at 1 μg/mL (100 μL/well) can bind JEV E-D3 antibody ,Mouse IgG with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
背景（Background）
Japanese encephalitis B, a mosquito-borne flavivirus, or B encephalitis, is the most important cause of viral encephalitis in Asia in terms of frequency and severity. JEV envelope protein E is an important research target, binding to host cell surface receptors and mediating fusion between the virus and the cell membrane.
