HRSV Post-Fusion glycoprotein F0 Specific ELISA Kit(For Vaccine Development)

背景（Background）

Most in vitro RSV neutralizing antibodies in human sera are directed against the prefusion conformation, but due to its instability the prefusion conformation has a propensity to prematurely refold into the stable postfusion conformation, both in solution and on the surface of the virions.

An RSV F protein that has both high expression levels and maintains a stable prefusion conformation would, therefore, be a promising subunit vaccine candidate against RSV.

To facilitate the RSV-related research, drug trials and vaccine development,a rapid and effective assay kit detecting the specific levels of HRSV Post-Fusion glycoprotein F0 is urgently needed to accelerate the development of RSV vaccines.

应用说明（Application）

This kit is developed for Specific quantitative detection of HRSV Post-Fusion glycoprotein F0 in samples.

This kit can specifically identify HRSV (A) and HRSV (B) Post-Fusion glycoprotein F0 by testing.

It is for research use only.

重构方法（Reconstitution）

Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

存储（Storage）

1. Unopened kit should be stored at 2℃-8℃ upon receiving.

2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

原理（Assay Principles）

This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of Pre-Fusion glycoprotein F0 (RSV). The kit consists of Pre-coated Anti-Pre-Fusion glycoprotein F0 (RSV) Antibody Microplate and Pre-Fusion glycoprotein F0 (RSV) Standard,HRP-Anti-Pre-Fusion glycoprotein F0 (RSV) Antibody and buffers.

Your experiment will include 5 simple steps:

a) Bring all reagents and samples to room temperature (20℃-25℃) before use.

b) Add your sample to the plate, take the Post-Fusion glycoprotein F0 (RSV) as Control sample. The samples and Control sample are diluted by Dilution Buffer.

c) Add the HRP-Anti-Post-Fusion glycoprotein F0 (RSV) Antibody diluted by Dilution Buffer to the plate.

d) Wash the plate and add TMB.

e) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.