膜杰作
Star Staining
产品描述（Product Details）
| Assay Type | Sandwich-ELISA |
| Analyte | Soluble Mesothelin |
| Format | 96T |
| Reactivity | Human |
| Regulatory Status | PRO |
| Sensitivity | 70.0 pg/mL |
| Standard Curve Range | 156.25 pg/mL-10000 pg/mL |
| Assay Time | 2 hr 45 min |
| Suitable Sample Type | For the quantitative determination of human Soluble Mesothelin in Cell Culture Supernatants, Plasma, Serum. |
| Sample volume | 50 μL |
组分（Materials Provided）
| ID | Components | Size |
| CEA030-C01 | Pre-coated Anti-Soluble Mesothelin Antibody Microplate | 1 plate |
| CEA030-C02 | Human Soluble Mesothelin Standard | 20 μg ×2 |
| CEA030-C03 | Biotin-Anti-Soluble Mesothelin Antibody Con. Solution | 100 μL |
| CEA030-C04 | Biotin-Antibody Dilution Buffer | 8 mL |
| CEA030-C05 | Streptavidin-HRP Con. Solution | 500 μL |
| CEA030-C06 | Streptavidin-HRP Dilution Buffer | 15 mL |
| CEA030-C07 | 20× Washing Buffer | 50 mL |
| CEA030-C08 | 2×Sample Dilution Buffer | 15 mL ×2 |
| CEA030-C09 | Substrate Solution | 12 mL |
| CEA030-C10 | Stop Solution | 6 mL |
背景（Background）
ClinMax™ ELISA Kit is convenient ready-to-use immunoassay Kit, specifically designed to quantitate human Mesothelin that is present in complex biological samples, such as human serum, plasma, and cell culture supernates.
A comprehensive validation of the ELISA method was performed following the ICH M10 on bioanalytical method validation and the FDA’s bioanalytical method validation guidance for industry. This validation included assessments of linearity, accuracy, precision, dilution linearity, recovery, and the hook effect. For details information, please refer to the DS.
ClinMax™ ELISA Kits are manufactured in a GMP-certified facility and comply to the ISO 13485 standard, ensuring a high level of quality and reliability.
应用说明（Application）
The kit is developed for quantitative detection of human soluble Mesothelin in serum, plasma and cell culture supernates.
It is for research use only.
存储（Storage）
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
原理（Assay Principles）
典型数据-Typical Data Please refer to Ds document for the assay protocol.
For each experiment, each ELISA plate needs to set the standard curve. The minimum detectable concentration of Soluble Mesothelin is less than 70.0 pg/mL.
背景（Background）：Mesothelin
Mesothelin (MSLN) is also known as CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, which belongs to the mesothelin family. Mesothelin / MSLN can be proteolytically cleaved into the following two chains by a furin-like convertase: Megakaryocyte-potentiating factor (MPF) and the cleaved form of mesothelin. Both MPF and the cleaved form of mesothelin are N-glycosylated. Mesothelin / MSLN can interacts with MUC16. The membrane-anchored forms of MSLN may play a role in cellular adhesion. MPF potentiates megakaryocyte colony formation in vitro.
