膜杰作
Star Staining
抗体来源（Source）
Monoclonal Anti-Human IgM mu Chain Antibody-Acridinium ester (M3HC) is a chimeric monoclonal antibody recombinantly expressed from human 293 cells (HEK293), which combines the variable region of a mouse monoclonal antibody with human IgG1 constant domain. The mouse monoclonal antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with Anti-Human IgM.
亚型（Isotype）
Human IgG1/kappa
特异性（Specificity）
This product is a specific antibody specifically reacts with Anti-Human IgM.
标记（Labeling）
Acridinium ester, can react with the primary amino group of protein. Under alkaline conditions, NHS is replaced as the leaving group, and the protein forms a stable amide bond with Acridinium ester.
蛋白标记度（Protein Ratio）
Passed as determined by binding MPCLIA.
应用（Application）
MPCLIA
纯度（Purity）
>90% as determined by SDS-PAGE.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH6.3 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Monoclonal Anti-Human IgM mu Chain Antibody-Acridinium ester (M3HC) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Monoclonal Anti-Human IgM mu Chain Antibody-Acridinium ester (M3HC) (Cat. No. AHM-S300) is more than 0.95 and the molecular weight of this protein is around 140-160 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-MPCLIA
Immobilized 0.04 μg /Test of Biotinylated SARS-CoV-2 Spike RBD, His,Avitag(Cat. No.SPD-C82E9) to the Streptavidin-Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A006) (10 μg beads/Test), incubated with 100 μL /Test of Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Chimeric mAb, Human IgM (AM122) (Cat. No. SPD-M162) at increasing concentration coupled to the Monoclonal Anti-Human IgM mu Chain Antibody-Acridinium ester (M3HC) (MALS verified) (Cat. No. AHM-S300, 0.04 μg /Test). Detection was performed with sensitivity of 0.488 ng/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (QC tested).
Protocol
The MPCLIA assay shows that Monoclonal Anti-Human IgM mu Chain Antibody-Acridinium ester (M3HC) (MALS verified) (Cat. No. AHM-S300) is stable at 37°C for 48 hours.
The MPCLIA assay shows that Monoclonal Anti-Human IgM mu Chain Antibody-Acridinium ester (M3HC) (MALS verified) (Cat. No. AHM-S300) is stable after freezing and thawing 3 times.
背景（Background）
The Anti-Human IgM-coupled Magnetic Beads are 2.8 µm superparamagnetic particles covalently coupled to a highly affinity Monoclonal anti-human IgM antibody. The beads can be used to capture the Human IgM in Chemiluminescence procedures. It has no-corss-reactivity with Human IgG, IgA or IgE. The Anti-Human IgM-coupled Magnetic Beads is easy to capture the human IgM, and the bounded antibody have no activity lost, this ready to use products could greatly save your protein coupling time and hassle, and help us get the best performance and highly reproducible results.
