膜杰作
Star Staining
抗体来源（Source）
Monoclonal Anti-Human-IgG-Fc Antibody, Mouse IgG1 is a chimeric monoclonal antibody recombinantly expressed from CHO cells, which combines the variable region of a mouse monoclonal antibody with mouse IgG1 constant domain. The mouse monoclonal antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with IgG Fc.
亚型（Isotype）
Mouse IgG1 | Kappa
特异性（Specificity）
This product is a specific antibody specifically reacts with IgG Fc.
应用（Application）
ELISA
纯度（Purity）
>95% as determined by SDS-PAGE.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20 to -70°C for 12 months in lyophilized state from date of receipt;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Monoclonal Anti-Human-IgG-Fc Antibody, Mouse IgG1 on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG4 (AM359b) (MALS verified) (Cat. No. SPD-M402a) at 2μg/mL (100μL/well) can bind Monoclonal Anti-Human-IgG-Fc Antibody, Mouse IgG1 (6F11C8)(Cat. No. IGG-S307) with a linear range of 0.05-3.13 ng/mL (QC tested).
Protocol
Immobilized Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG2 (AM359b) (MALS verified) (Cat. No. SPD-M400a), Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG3 (AM359b) (MALS verified) (Cat. No. SPD-M401a), Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG4 (AM359b) (MALS verified) (Cat. No. SPD-M402a) and Human ACE2 / ACEH Protein, Fc Tag (MALS verified) (Cat. No. AC2-H5257) can bind Monoclonal Anti-Human-IgG-Fc Antibody, Mouse IgG1 (6F11C8)(Cat. No. IGG-S307). The antibody does not bind Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgA1 (AM130) (MALS verified) (Cat. No. S1N-M164) and Human CD19 (20-291) Protein, His Tag DMF Filed (Cat. No. CD9-H52H2) (Routinely tested).
Protocol
背景（Background）
Crystallizable fragments composed of the carboxy-terminal halves of both IMMUNOGLOBULIN HEAVY CHAINS linked to each other by disulfide bonds. Fc fragments contain the carboxy-terminal parts of the heavy chain constant regions that are responsible for the effector functions of an immunoglobulin (COMPLEMENT fixation, binding to the cell membrane via FC RECEPTORS, and placental transport).
