膜杰作
Star Staining
抗体来源（Source）
Monoclonal Anti-HSV-2-Glycoprotein D Antibody, Human IgG1 (3H9) is a chimeric monoclonal antibody recombinantly expressed from human 293 cells (HEK293), which combines the variable region of a mouse monoclonal antibody with human IgG1 constant domain. The mouse monoclonal antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with Glycoprotein D (HSV-2).
亚型（Isotype）
Human IgG1 | Human Kappa
特异性（Specificity）
This product is a specific antibody specifically reacts with Glycoprotein D (HSV-2).
应用（Application）
ELISA
纯度（Purity）
>90% as determined by SDS-PAGE.
内毒素（Endotoxin）
Less than 1.0 EU per mg by the LAL method.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20 to -70°C for 12 months in lyophilized state from date of receipt;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Monoclonal Anti-HSV-2-Glycoprotein D Antibody, Human IgG1 (3H9) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized HSV-1 (strain 17) Envelope Glycoprotein D (gD), His Tag (Cat. No. GLD-V52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-HSV-2-Glycoprotein D Antibody, Human IgG1 (3H9) (Cat. No. GLD-M609) with a linear range of 1-31 ng/mL (QC tested).
Protocol
Immobilized HSV-2 (strain HG52) Envelope Glycoprotein D (gD), His Tag (Cat. No. GLD-V52H4) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-HSV-2-Glycoprotein D Antibody, Human IgG1 (3H9) (Cat. No. GLD-M609) with a linear range of 0.1-2 ng/mL (Routinely tested).
Protocol
背景（Background）
Herpesvirus infections are widely spread throughout the world population. Herpes simplex virus (HSV) belongs to the α-herpesvirus subfamily. There are two main types of HSV, HSV-1 and HSV-2, which infect humans. HSV-2 mainly causes genital lesions, whereas HSV-1 is involved in both oral and genital infections. Glycoprotein D (gD) is a structural component of the herpes simplex virus type 1 (HSV-1) envelope which is essential for virus entry and fusion with host cells. gD plays an important role by binding to the host receptors such as herpes virus entry mediator (HVEM) and nectin-1, a member of the immunoglobulin (Ig)-like cell adhesion molecules.
