膜杰作
Star Staining
产品描述（Product Details）
| Assay Type | Sandwich-ELISA |
| Analyte | NKp46 |
| Format | 96T |
| Reactivity | Human |
| Sensitivity | 15.0 pg/mL |
| Standard Curve Range | 15.625 pg/mL-1000 pg/mL |
| Assay Time | 2 hr 45 min |
| Suitable Sample Type | For the quantitative determination of human NKp46 in Cell Culture Supernatants, Plasma, Serum. |
| Sample volume | 100 μL |
组分（Materials Provided）
| ID | Components | Size |
| CEA040-B01 | Pre-coated Anti-NKp46 Antibody Microplate | 1 plate |
| CEA040-B02 | Human NKp46 Standard | 20 μg ×2 |
| CEA040-B03 | Biotin-Anti-NKp46 Antibody Con. Solution | 200 μL |
| CEA040-B04 | 2×Biotin-Antibody Dilution Buffer | 8mL |
| CEA040-B05 | Streptavidin-HRP Con. Solution | 0.5 mL×2 |
| CEA040-B06 | Streptavidin-HRP Dilution Buffer | 15 mL |
| CEA040-B07 | 20× Washing Buffer | 50 mL |
| CEA040-B08 | Sample Dilution Buffer | 15 mL ×2 |
| CEA040-B09 | Substrate Solution | 12 mL |
| CEA040-B10 | Stop Solution | 6 mL |
背景（Background）
ClinMax™ ELISA Kit is convenient ready-to-use immunoassay Kit, specifically designed to quantitate human NKp46/NCR1 that is present in complex biological samples, such as human serum, plasma, and cell culture supernates.
A comprehensive validation of the ELISA method was performed following the ICH M10 on bioanalytical method validation and the FDA’s bioanalytical method validation guidance for industry. This validation included assessments of linearity, accuracy, precision, dilution linearity, recovery, and the hook effect. For details information, please refer to the DS.
ClinMax™ ELISA Kits are manufactured in a GMP-certified facility and comply to the ISO 13485 standard, ensuring a high level of quality and reliability.
应用说明（Application）
The kit is developed for quantitative detection of human NKp46 in serum, plasma and cell culture supernates.
It is for research use only.
存储（Storage）
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
原理（Assay Principles）
典型数据-Typical Data Please refer to DS document for the assay protocol.
For each experiment, each ELISA plate needs to set the standard curve. The minimum detectable concentration of CEA040-B02 is less than 15.0 pg/mL.
背景（Background）：NKp46
Natural cytotoxicity triggering receptor 1 (NCR1) is also known as Natural killer cell p46-related protein (NK-p46), Lymphocyte antigen 94 homolog (LY94), CD antigen CD335, which belongs to the natural cytotoxicity receptor (NCR) family. NCR1 contains two Ig-like (immunoglobulin-like) domains. NCR1 interacts with CD247 and FCER1G. NCR1 / CD335 may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis.
