分子别名(Synonym)
CD258 antigen,CD258,HVEM-L,LIGHT,LTg,TNFSF14,TR2,HVEML
表达区间及表达系统(Source)
Cynomolgus LIGHT Protein, His Tag (LIT-C5244) is expressed from human 293 cells (HEK293). It contains AA Asp 74 - Val 240 (Accession # E9NVF7).
Predicted N-terminus: His
蛋白结构(Molecular Characterization)
This protein carries a polyhistidine tag at the N-terminus
The protein has a calculated MW of 58.6 kDa. The protein migrates as 63-66 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳(SDS-PAGE)
Cynomolgus LIGHT Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Cynomolgus LIGHT Protein, His Tag (Cat. No. LIT-C5244) is more than 90% and the molecular weight of this protein is around 55-70 kDa verified by SEC-MALS.
Report
活性(Bioactivity)-ELISA
Immobilized Human DcR3, Fc Tag (Cat. No. TNB-H5255) at 5 μg/mL (100 μL/well) can bind Cynomolgus LIGHT Protein, His Tag (Cat. No. LIT-C5244) with a linear range of 2-39 ng/mL (QC tested).
Protocol
Immobilized Cynomolgus LIGHT Protein, His Tag (Cat. No. LIT-C5244) at 2 μg/mL (100 μL/well) can bind Human HVEM, Fc Tag (Cat. No. HVM-H5258) with a linear range of 0.1-4 ng/mL (Routinely tested).
Protocol
背景(Background)
Tumor necrosis factor ligand superfamily member 14(LIGHT) is a tumor necrosis factor (TNF) superfamily ligand that regulates T cell immune responses by signaling through the herpes virus entry mediator (HVEM) and the lymphotoxin beta receptor (LTbetaR). LIGHT has emerged as a potent initiator of T cell co-stimulation signals effecting CTL-mediated tumor rejection, allograft rejection and graft versus host disease. Constitutive expression of LIGHT leads to tissue destruction and autoimmune-like disease syndromes.