膜杰作
Star Staining
分子别名（Synonym）
IL5Ra,CD125
表达区间及表达系统（Source）
Biotinylated Human IL-5 R alpha Protein, Avitag,Fc Tag (ILA-H82F5) is expressed from human 293 cells (HEK293). It contains AA Asp 21 - Trp 342 (Accession # Q01344-1).
Predicted N-terminus: Gly
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a human IgG1 Fc tag.
The protein has a calculated MW of 64.9 kDa. The protein migrates as 70-90 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Biotinylated Human IL-5 R alpha Protein, Avitag,Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human IL-5 R alpha Protein, Avitag,Fc Tag (Cat. No. ILA-H82F5) is more than 85% and the molecular weight of this protein is around 150-180 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human IL-5, His Tag, premium grade (Cat. No. IL5-H52H3) at 10 μg/mL (100 μL/well) can bind Biotinylated Human IL-5 R alpha Protein, Avitag,Fc Tag (Cat. No. ILA-H82F5) with a linear range of 0.1-10 ng/mL (QC tested).
Protocol
背景（Background）
Interleukin-5 receptor subunit alpha (IL5RA) is also known as IL-5 receptor subunit alpha, IL-5R subunit alpha and CD125,which is a single-pass type I membrane protein that belongs to the type I cytokine receptor family and type 5 subfamily.IL5R, a heterodimer of an alpha and a beta subunit, is expressed on eosinophils and basophils. IL5RA / CD125 is the receptor for interleukin-5 (IL5) and the alpha chain can bind to IL5. The WSXWS motif of IL5RA appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.The beta subunit of IL5R is common to the IL3, IL5 and GM-CSF receptors.
