膜杰作
Star Staining
描述（Description）
HEK293/Human Alpha-synuclein (GFP) Stable Cell Line
应用说明（Application）
α-Synuclein PFF induced intracellular aggregation assay
生长特性（Growth Properties）
Adherent
筛选标记（Selection Marker）
Hygromycin B (250 μg/mL)
培养基（Culture Medium）
DMEM medium + 10% FBS
冻存液（Freeze Medium）
10% DMSO + 90% FBS
装量（Quantity）
1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
存储（Storage）
Frozen in liquid nitrogen.
支原体检测（Mycoplasma Testing）
Negative
无菌检测（Sterility Testing）
Negative
使用说明（Instructions for Use）
See data sheet for detailed culturing and assay protocol.
活性（Bioactivity）
FACS analysis of Alpha-synuclein (GFP) on HEK293/Human Alpha-synuclein (GFP) Stable Cell Line.
HEK293/Human Alpha-synuclein (GFP) Stable Cell Line was red line, negative control HEK293 cells was grey line (QC tested).
Protocol
活性（Bioactivity）-Bioactivity CELL BASE
HEK293/Human Alpha-Synuclein (GFP) Stable Cell Line (Cat. No. CHEK-ATP085) were transduced with Human Alpha-Synuclein Pre-formed Fibrils, His Tag and Human Alpha-Synuclein, His Tag (Cat. No. ALN-H52H8) respectively. The fluorescence of GFP-Alpha-Synuclein (Green) and DAPI (Blue) were detected by confocal microscope. A. Lipo2000 transduction. B. Lipo2000 and Human Alpha-Synuclein, His Tag transduction. C. Lipo2000 and Human Alpha-Synuclein Pre-formed Fibrils, His Tag transduction. Scale bars, 50 μm (Routinely tested).
Protocol
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背景（Background）
Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Alternatively spliced transcripts encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2016]
License Disclosure
This cell line is provided for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit modification of this cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license. Modifications of this cell line, transfer to another facility, or commercial use of the cell lines may require a separate license and additional fees. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.
