膜杰作
Star Staining
抗体来源（Source）
Biotinylated Anti-Adalimumab Antibody (22E7A4A2) (ADB-BY17c) is a monoclonal antibody recombinantly expressed from human 293 cells (HEK293). The mouse monoclonal antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with Adalimumab ADA.
亚型（Isotype）
Mouse IgG1/kappa
特异性（Specificity）
Recognizes Adalimumab specifically, no cross reactivity with other humanized antibodies.
标记（Labeling）
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A specially optimized long-spacer biotin reagent (13.5 angstroms) is used in this product to minimize potential steric hindrance.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- 4-8°C for 12 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Biotinylated Anti-Adalimumab Antibody (22E7A4A2) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Biotinylated Anti-Adalimumab Antibody (22E7A4A2) (Cat. No. ADB-BY17c) is more than 90% and the molecular weight of this protein is around 135-155 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Adalimumab at 1 μg/mL (100 μL/well) can bind Biotinylated Anti-Adalimumab Antibody (22E7A4A2) (Cat. No. ADB-BY17c) with a linear range of 0.4-3 ng/mL (QC tested).
Protocol
背景（Background）
Adalimumab is a human monoclonal antibody against TNF-alpha. It is produced by recombinant D technology using a mammalian cell expression system. It consists of 1330 amino acids and has a molecular weight of approximately 148 kilodaltons.
