膜杰作
Star Staining
分子别名（Synonym）
FCGR2B,C,CD32b,c,FcRII-b,c,Fc-gamma RII-b,c,Fc-gamma-RIIb,c,CD32,FCG2,IGFR2,CDw32
表达区间及表达系统（Source）
Human CD32b/c Protein, Strep Tag (CDB-H5284) is expressed from human 293 cells (HEK293). It contains AA Ala 46 - Pro 217 (Accession # P31994-1).
Predicted N-terminus: Ala 46
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a twin strep tag at the C-terminus
The protein has a calculated MW of 22.7 kDa. The protein migrates as 30-34 kDa under reducing (R) condition (SDS-PAGE).
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human CD32b/c Protein, Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human CD32b/c Protein, Strep Tag (Cat. No. CDB-H5284) is more than 95% and the molecular weight of this protein is around 26-38 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-SPR
Human CD32b/c Protein, Strep Tag (Cat. No. CDB-H5284) immobilized on CM5 Chip can bind Rituximab with an affinity constant of 10.2 μM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
Rituximab immobilized on CM5 Chip can bind Human CD32b/c Protein, Strep Tag (Cat. No. CDB-H5284) with an affinity constant of 5.18 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
背景（Background）
Receptors for the Fc region of IgG (Fc γ R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc γ Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized.
There are three genes for human Fcγ RII /CD32 (A, B, and C) and one for mouse Fcγ RII B (CD32B). CD32 is a low affinity receptor for IgG. Low affinity immunoglobulin gamma Fc region receptor II-b (FCGR2B) is also known as CD32b, FCG2, IGFR2. CD32B is expressed on B cells and myeloid dendritic cells. Ligation of CD32B on B cells downregulates antibody production and may, in some circumstances, promote apoptosis. Co-ligation of CD32B on dendritic cells inhibits maturation and blocks cell activation. CD32B may also be a target for monoclonal antibody therapy for malignancies.
