膜杰作
Star Staining
表达区间及表达系统（Source）
Human Matriptase / ST14 Catalytic Domain Protein, His Tag (MAE-H5143) is expressed from E. coli cells. It contains AA Gly 596 - Val 855 (Accession # NP_068813).
Predicted N-terminus: Met
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 30.6 kDa. The protein migrates as 27-28 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Supplied as 0.2 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
电泳（SDS-PAGE）
Human Matriptase / ST14 Catalytic Domain Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）
Measured by its ability to cleave the fluorogenic peptide substrate Boc-QAR-AMC . The specific activity is >20,000 pmol/min/μg (QC tested).
Protocol
背景（Background）
Can degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase, Similar to stromelysin 1, but action on collagen types III, IV and V is weak.
