膜杰作
Star Staining
分子别名（Synonym）
Carbonic Anhydrase XII,CA XII,Car12,CA-XII,Carbonate dehydratase XII
表达区间及表达系统（Source）
Biotinylated Cynomolgus Carbonic Anhydrase XII Protein, His,Avitag (CAI-C82E3) is expressed from human 293 cells (HEK293). It contains AA Ala 25 - Gly 299 (Accession # A0A2K5TSB2-1).
Predicted N-terminus: Ala 25
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)
The protein has a calculated MW of 34.5 kDa. The protein migrates as 42-52 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 50mM Tris,150mM NaCl,PH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Biotinylated Cynomolgus Carbonic Anhydrase XII Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Biotinylated Cynomolgus Carbonic Anhydrase XII Protein, His,Avitag (Cat. No. CAI-C82E3) is more than 95% and the molecular weight of this protein is around 45-60 kDa verified by SEC-MALS.
Report
活性（Bioactivity）
Measured by its esterase activity. The specific activity is >10 pmol/min/μg (QC tested).
Protocol
背景（Background）
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid.
