膜杰作
Star Staining
分子别名（Synonym）
AP-TNAP, APTNAP, HOPS, HPPA, HPPC, HPPI, HPPO, TNALP, TNAP, TNS-ALP, TNSALP
表达区间及表达系统（Source）
Human ALPL Protein, His Tag (ALL-H52H3) is expressed from human 293 cells (HEK293). It contains AA Leu 18 - Ser 502 (Accession # P05186-1).
Predicted N-terminus: Leu 18
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 55.3 kDa. The protein migrates as 65-70 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Supplied as 0.2 μm filtered solution in 20 mM Tris, 150 mM NaCl, 20% Glycerol, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
电泳（SDS-PAGE）
Human ALPL Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human ALPL Protein, His Tag (Cat. No. ALL-H52H3) is more than 90% and the molecular weight of this protein is around 115-135 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human ALPL Protein, His Tag (Cat. No. ALL-H52H3) at 1 μg/mL (100 μL/well) can bind various dilution ratio of Anti-ALPL Polyclonal Antibody (QC tested).
Protocol
活性（Bioactivity）
Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP). The specific activity is >80,000 pmol/min/μg (QC tested).
Protocol
背景（Background）
This gene encodes a member of the alkaline phosphatase family of proteins. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme may play a role in bone mineralization. Mutations in this gene have been linked to hypophosphatasia, a disorder that is characterized by hypercalcemia and skeletal defects.
