膜杰作
Star Staining
分子别名（Synonym）
HN protein
表达区间及表达系统（Source）
Mumps virus (strain Miyahara vaccine) (MuV) HN Protein, His Tag (HNN-M52H3) is expressed from human 293 cells (HEK293). It contains AA Gly 106 - Thr 582 (Accession # P11235).
Predicted N-terminus: Gly 106
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 54.6 kDa. The protein migrates as 66-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Mumps virus (strain Miyahara vaccine) (MuV) HN Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Mumps virus (strain Miyahara vaccine) (MuV) HN Protein, His Tag (Cat. No. HNN-M52H3) is more than 90% and the molecular weight of this protein is around 63-78 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-SPR
Mumps virus (strain Miyahara vaccine) (MuV) HN Protein, His Tag (Cat. No. HNN-M52H3) immobilized on CM5 Chip can bind 3'-Sialyllactose / GM3 Ganglioside oligosaccharide with an affinity constant of 586 μM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
背景（Background）
Mumps immunity is typically assessed by measuring neutralizing-antibody responses directed against mumps HN and F proteins.
