膜杰作
Star Staining
分子别名（Synonym）
Coagulation factor XI,FXI,PTA,F11
表达区间及表达系统（Source）
Rhesus macaque Coagulation factor XI Protein, His Tag (FXI-C52H3) is expressed from human 293 cells (HEK293). It contains AA Glu 19 - Ala 624 (Accession # G7MSF8-1).
Predicted N-terminus: Glu 19
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 69.8 kDa. The protein migrates as 80-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 20 mM HEPES,150 mM NaCl,pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Rhesus macaque Coagulation factor XI Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
活性（Bioactivity）-ELISA
Immobilized Rhesus Coagulation factor XI, His Tag (Cat. No. FXI-C52H3) at 1 μg/mL (100 μL/well) can bind Abelacimab with a linear range of 0.1-1 ng/mL (QC tested).
Protocol
活性（Bioactivity）-SPR
Abelacimab captured on Protein A Chip can bind Rhesus macaque Coagulation factor XI, His Tag (Cat. No. FXI-C52H3) with an affinity constant of 22.4 pM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
背景（Background）
This protein is present in plasma as a zymogen, which is a unique plasma coagulation enzyme because it exists as a homodimer consisting of two identical polypeptide chains linked by disulfide bonds. During activation of the plasma factor XI, an internal peptide bond is cleaved by factor XIIa (or XII) in each of the two chains, resulting in activated factor XIa, a serine protease composed of two heavy and two light chains held together by disulfide bonds. This activated plasma factor XI triggers the middle phase of the intrisic pathway of blood coagulation by activating factor IX. Defects in this factor lead to Rosenthal syndrome, a blood coagulation abnormality.
