膜杰作
Star Staining
分子别名（Synonym）
BGCAN, betaglycan
表达区间及表达系统（Source）
Human TGF-beta RIII / TGFBR3 Protein, His Tag (TG3-H52H3) is expressed from human 293 cells (HEK293). It contains AA Gly 21 - Val 787 (Accession # Q03167-1).
Predicted N-terminus: Gly 21
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 86.7 kDa. The protein migrates as 100 kDa and 130-170 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human TGF-beta RIII / TGFBR3 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-BLI
Loaded Biotinylated Human TGF-Beta 1, Avitag (Cat. No. TG1-H8217) on SA Biosensor, can bind Human TGF-beta RIII / TGFBR3 Protein, His Tag (Cat. No. TG3-H52H3) with an affinity constant of 1.80 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Protocol
背景（Background）
This locus encodes the transforming growth factor (TGF)-beta type III receptor. The encoded receptor is a membrane proteoglycan that often functions as a co-receptor with other TGF-beta receptor superfamily members. Ectodomain shedding produces soluble TGFBR3, which may inhibit TGFB signaling. Decreased expression of this receptor has been observed in various cancers. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
