膜杰作
Star Staining
分子别名（Synonym）
Cadherin-17,CDH17,HPT-1, LI-cadherin
表达区间及表达系统（Source）
Human Cadherin-17 Protein, Mouse IgG1 Fc Tag (CA7-H52A9) is expressed from human 293 cells (HEK293). It contains AA Gln 23 - Met 787 (Accession # Q12864-1).
Predicted N-terminus: Gln 23
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a mouse IgG1 Fc tag at the C-terminus
The protein has a calculated MW of 111.2 kDa. The protein migrates as 130-135 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 150 mM NaCl, pH8.0 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human Cadherin-17 Protein, Mouse IgG1 Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
活性（Bioactivity）-ELISA
Immobilized Human Cadherin-17 Protein, Mouse IgG1 Fc Tag (Cat. No. CA7-H52A9) at 1 μg/mL (100 μL/well) can bind Human Anti-Cadherin 17 with a linear range of 0.5-31 ng/mL (QC tested).
Protocol
背景（Background）
Cadherin-17, also known as liver-intestine (LI) Cadherin, belongs to the cadherin family of calcium-dependent cell adhesion molecules. In vivo studies showed CDH17 knockout resulted in apoptotic PC tumor death through activating caspase-3 activity. Taken together, CDH17 functions as an oncogenic molecule critical to PC growth by regulating tumor apoptosis signaling pathways and CDH17 could be targeted to develop an anti-PC therapeutic approach.
